abstract：:The production and characterization of an active recombinant N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in Escherichia coli BL21(DE3) has been previously reported. In this study, the effect of the signal peptide (SP), inducer concentration, process scale, and operational mode (batch and semi-continuous) on GALN...

journal_title：Journal of microbiology and biotechnology

authors： Hernández A,Velásquez O,Leonardi F,Soto C,Rodríguez A,Lizaraso L,Mosquera Á,Bohórquez J,Coronado A,Espejo Á,Sierra R,Sánchez OF,Alméciga-Díaz CJ,Barrera LA

更新日期：2013-05-01 00:00:00

abstract：:Helicobacter pylori increased the gamma-glutamyltranspeptidase (GGT) production under low-pH (maximal at pH 4) and appropriate pCO2 conditions, while the production of GGT mRNA correlated with increased total enzyme activity. At pH 4, the bacterium augmented enzyme production in the presence of glutamine (~10 mM) in t...

journal_title：Journal of microbiology and biotechnology

authors： Ki MR,Yun NR,Hwang SY

更新日期：2013-04-01 00:00:00

abstract：:Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,124 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative re...

journal_title：Journal of microbiology and biotechnology

authors： Kim SY,Oh CG,Lee YJ,Choi KH,Shin DS,Lee SK,Park KJ,Shin H,Park MS,Lee JH

更新日期：2013-04-01 00:00:00

abstract：:Cryptomonads are unicellular, biflagellate algae. Generally, cryptomonad cells cannot be preserved well because of their fragile nature, and an improved methodology should be developed to identify cryptomonads from natural habitats. In this study, we tried using several cytological fixatives, including glutaraldehyde,...

journal_title：Journal of microbiology and biotechnology

authors： Xia S,Cheng Y,Zhu H,Liu G,Hu Z

更新日期：2013-03-01 00:00:00

abstract：:Monacolin K and yellow pigment, produced by Monascus sp., have each been proven to be beneficial compounds as antihypercholesterolemic and anti-inflammation agents, respectively. However, citrinin, a human toxic substance, was also synthesized in this fungus. In this research, solidstate fermentation of M. purpureus T...

journal_title：Journal of microbiology and biotechnology

authors： Jirasatid S,Nopharatana M,Kitsubun P,Vichitsoonthonkul T,Tongta A

更新日期：2013-03-01 00:00:00

abstract：:In the continued search for inhibitors of enoyl-acyl carrier protein (ACP) reductase, we found that four acylbenzenediol sulfate metabolites from Streptomyces sp. AN1761 potently inhibited bacterial enoyl-ACP reductases of Staphylococcus aureus, Streptococcus pneumoniae, and Mycobacterium tuberculosis. Their structure...

journal_title：Journal of microbiology and biotechnology

authors： Kwon YJ,Sohn MJ,Oh T,Cho SN,Kim CJ,Kim WG

更新日期：2013-02-01 00:00:00

abstract：:Rhodococcus sp. strain p52, a previously isolated dibenzofuran degrader, could effectively inhibit the growth of cyanobacteria, including species of Microcystis, Anabaena, and Nodularia. When strain p52 was inoculated at the concentration of 7.7×10(7) CFU/ml, 93.5% of exponentially growing Microcystis aeruginosa (7.3×...

journal_title：Journal of microbiology and biotechnology

authors： Wang MH,Peng P,Liu YM,Jia RB,Li L

更新日期：2013-02-01 00:00:00

abstract：:Tetanus is a specific infectious disease, which is often associated with catastrophic events such as earthquakes, traumas, and war wounds. The obligate anaerobe Clostridium tetani is the pathogen that causes tetanus. Once the infection of tetanus progresses to an advanced stage within the wounds of limbs, the rates of...

journal_title：Journal of microbiology and biotechnology

authors： Jiang D,Pu X,Wu J,Li M,Liu P

更新日期：2013-01-01 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:Genus Bacillus is a spore-forming bacterium that has unique properties in cell differentiation, allowing the forming of spores in stress conditions and activated in the vegetative cell, with suitable environments occurring during the life cycle acting as a trigger. Their habitat is mainly in soil; thus, many species o...

journal_title：Journal of microbiology and biotechnology

authors： Mongkolthanaruk W

更新日期：2012-12-01 00:00:00

abstract：:In this study, DEAE-corncobs [delignified corncob grits derivatized with 2-(diethylamino)ethyl chloride hydrochloride (DEAE·HCl)] were prepared as a carrier to immobilize yeast (Saccharomyces cerevisiae) for ethanol production. The immobilized yeast cell reactor produced ethanol under optimized DEAE·HCl derivatization...

journal_title：Journal of microbiology and biotechnology

authors： Lee SE,Lee CG,Kang DH,Lee HY,Jung KH

更新日期：2012-12-01 00:00:00

abstract：:An extensive investigation was carried out to describe the kinetics of cell growth, substrate consumption, and product formation in the batch fermentation using starch as substrate. Evaluation of intrinsic kinetic parameters was carried out using a best-fit unstructured model. A nonlinear regression technique was appl...

journal_title：Journal of microbiology and biotechnology

authors： Bhunia B,Basak B,Bhattacharya P,Dey A

更新日期：2012-12-01 00:00:00

abstract：:Fermentation with filamentous fungi in a bioreactor is a complex dynamic process that is affected by flow conditions and the evolution of the rheological properties of the medium. These properties are mainly affected by the biomass concentration and the morphology of the fungus. In this work, the rheological propertie...

journal_title：Journal of microbiology and biotechnology

authors： Núñez-Ramírez DM,Medina-Torres L,Valencia-López JJ,Calderas F,López Miranda J,Medrano-Roldán H,Solís-Soto A

更新日期：2012-11-01 00:00:00

abstract：:Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268...

journal_title：Journal of microbiology and biotechnology

authors： Cho MS,Park DS,Lee JW,Chi HY,Sohn SI,Jeon BK,Ma JB

更新日期：2012-11-01 00:00:00

abstract：:In the present work, current knowledge on the potential fate, microbial degradation, and toxicity of hexahydro- 1,3,5-trinitro-1,3,5-triazine (RDX) was thoroughly reviewed, focusing on the toxicological assessment of a variety of potential RDX degradation pathways in bacteria and fungi. The present review on microbial...

journal_title：Journal of microbiology and biotechnology

authors： Khan MI,Lee J,Park J

更新日期：2012-10-01 00:00:00

abstract：:A xylanase-producing thermophilic strain, Geobacillus sp. TC-W7, was isolated from a hot spring in Yongtai (Fuzhou, China). Subsequently, the xylanase gene that encoded 407 amino acids was cloned and expressed. The recombinant xylanase was purified by GST affinity chromatography and exhibited maximum activity at 75 de...

journal_title：Journal of microbiology and biotechnology

authors： Liu B,Zhang N,Zhao C,Lin B,Xie L,Huang Y

更新日期：2012-10-01 00:00:00

abstract：:This study investigated the hydrocarbonoclastic microbial community present on weathered crude oil and their ability to degrade weathered oil in seawater obtained from the Gulf St. Vincent (SA, Australia). Examination of the native seawater communities capable of utilizing hydrocarbon as the sole carbon source identif...

journal_title：Journal of microbiology and biotechnology

authors： Sheppard PJ,Simons KL,Kadali KK,Patil SS,Ball AS

更新日期：2012-09-01 00:00:00

abstract：:A gene (acas) designated as alpha-amylase was cloned from Arthrobacter chlorophenolicus A6. The multiple amino acid sequence analysis and functional expression of acas revealed that this gene really encoded an amylosucrase (ASase) instead of alpha-amylase. In fact, the recombinant enzyme exhibited typical ASase activi...

journal_title：Journal of microbiology and biotechnology

authors： Seo DH,Jung JH,Choi HC,Cho HK,Kim HH,Ha SJ,Yoo SH,Cha J,Park CS

更新日期：2012-09-01 00:00:00

abstract：:In order to predict biologically significant attributes such as function from protein sequences, searching against large databases for homologous proteins is a common practice. In particular, BLAST and HMMER are widely used in a variety of biological fields. However, sequencehomologous proteins determined by BLAST and...

journal_title：Journal of microbiology and biotechnology

authors： Yu DS,Lee DH,Kim SK,Lee CH,Song JY,Kong EB,Kim JF

更新日期：2012-08-01 00:00:00

abstract：:Nargenicin A1 is a 28-membered polyketide macrolide, with antibacterial activity against methicillin-resistant Staphylococcus aureus, produced by Nocardia sp. CS682. In this study, the production of nargenicin A1 was improved by enhancing the supply of different biosynthetic precursors. In Nocardia sp. CS682 (KCTC1129...

journal_title：Journal of microbiology and biotechnology

authors： Koju D,Maharjan S,Dhakal D,Yoo JC,Sohng JK

更新日期：2012-08-01 00:00:00

abstract：:Homology modeling of Streptomyces peucetius CYP147F1 was constructed using three cytochrome P450 structures, CYP107L1, CYPVdh, and CYPeryF, as templates. The lowest energy SPCYP147F1 model was then assessed for stereochemical quality and side-chain environment by Accelrys Discovery Studio 3.1 software. Further actives...

journal_title：Journal of microbiology and biotechnology

authors： Bhattarai S,Liou K,Oh TJ

更新日期：2012-07-01 00:00:00

abstract：:Fungal biomass and fungal extract of the nonpathogenic fungus Neurospora crassa were successfully used as reducing agents for the biosynthesis of platinum nanoparticles (PtNPs). The experiment was carried out by exposing the fungal biomass or the fungal extract to a 0.001 M precursor solution of hexachloroplatinic(IV)...

journal_title：Journal of microbiology and biotechnology

authors： Castro-Longoria E,Moreno-Velázquez SD,Vilchis-Nestor AR,Arenas-Berumen E,Avalos-Borja M

更新日期：2012-07-01 00:00:00

abstract：:Copper-containing compounds are introduced into the environment through agricultural chemicals, mining, and metal industries and cause severe detrimental effects on ecosystems. Certain microorganisms exposed to these stressors exhibit molecular mechanisms to maintain intracellular copper homeostasis and avoid toxicity...

journal_title：Journal of microbiology and biotechnology

authors： Ng SP,Palombo EA,Bhave M

更新日期：2012-06-01 00:00:00

abstract：:Keratinases are exciting keratin-degrading enzymes; however, there have been relatively few studies on their immobilization. A keratinolytic protease from Chryseobacterium sp. kr6 was purified and its partial sequence determined using mass spectrometry. No significant homology to other microbial peptides in the NCBI d...

journal_title：Journal of microbiology and biotechnology

authors： Silveira ST,Gemelli S,Segalin J,Brandellli A

更新日期：2012-06-01 00:00:00

abstract：:The antagonistic effect of Trichoderma harzianum on a range of seed-borne fungal pathogens of wheat (viz. Fusarium graminearum, Bipolaris sorokiniana, Aspergillus spp., and Penicillium spp.) was assessed. The potential of T. harzianum as a biocontrol agent was tested in vitro and under field conditions. Coculture of t...

journal_title：Journal of microbiology and biotechnology

authors： Hasan MM,Rahman SM,Kim GH,Abdallah E,Oh DH

更新日期：2012-05-01 00:00:00

abstract：:Osteoarthritis (OA) is the most common rheumatic pathology. One of the major objectives of OA research is the development of early diagnostic strategies such as those using proteomic technology. Synovial fluid (SF) in OA patients is a potential source of biomarkers for OA. The efficient and reliable preparation of SF ...

journal_title：Journal of microbiology and biotechnology

authors： Kong MK,Min BH,Lee PC

更新日期：2012-05-01 00:00:00

abstract：:Thirty-seven carbofuran-degrading bacteria were isolated from agricultural soils, and their genetic and phenotypic characteristics were investigated. The isolates were able to utilize carbofuran as a sole source of carbon and energy. Analysis of the 16S rRNA gene sequence indicated that the isolates were related to me...

journal_title：Journal of microbiology and biotechnology

authors： Shin DH,Kim DU,Seong CN,Song HG,Ka JO

更新日期：2012-04-01 00:00:00

abstract：:The present study was aimed to investigate the effect of a probiotic, Enterococcus faecium, on the immune responses against infection with the marine fish pathogen Lactococcus garvieae in olive flounder (Paralichthys olivaceus). The immune responses were assessed by lysozyme activity, complement activity, protease act...

journal_title：Journal of microbiology and biotechnology

authors： Kim YR,Kim EY,Choi SY,Hossain MT,Oh R,Heo WS,Lee JM,Cho YC,Kong IS

更新日期：2012-04-01 00:00:00

abstract：:In this paper, the kinetics of a cloned special glucosidase, named ginsenosidase type III hydrolyzing 3-O-glucoside of multi-protopanaxadiol (PPD)-type ginsenosides, were investigated. The gene (bgpA) encoding this enzyme was cloned from a Terrabacter ginsenosidimutans strain and then expressed in E. coli cells. Ginse...

journal_title：Journal of microbiology and biotechnology

authors： Jin XF,Yu HS,Wang DM,Liu TQ,Liu CY,An DS,Im WT,Kim SG,Jin FX

更新日期：2012-03-01 00:00:00

abstract：:Previous studies have demonstrated that repeated restraint stress in rodents produces increases in depression and anxietylike behaviors and alters the expression of corticotrophinreleasing factor (CRF) in the hypothalamus. The current study focused on the impact of Bupleurum falcatum (BF) extract administration on rep...

journal_title：Journal of microbiology and biotechnology

authors： Lee B,Yun HY,Shim I,Lee H,Hahm DH

更新日期：2012-03-01 00:00:00

abstract：:Laccase- and peroxidase-free tyrosinase has commercial importance in the production of L-3, 4-dihydroxyphenylalanine (L-DOPA), which is mainly used in the treatment of Parkinson's disease. In the present study, isolation of an actinomycetes microbial strain capable of producing only tyrosinase is reported. Among all s...

journal_title：Journal of microbiology and biotechnology

authors： Sambasiva Rao KR,Tripathy NK,Mahalaxmi Y,Prakasham RS

更新日期：2012-02-01 00:00:00

abstract：:Recently, we reported that the synthetic Coprisin analog peptide 9-mer dimer CopA3 (consisted of all-L amino acid sequence) was designed based on a defensin-like peptide, Coprisin isolated from Copris tripartitus. The 9-mer dimer CopA3 (L-CopA3) had antibacterial activity and induced apoptosis in human leukemia cells ...

journal_title：Journal of microbiology and biotechnology

authors： Kim SJ,Kim IW,Kwon YN,Yun EY,Hwang JS

更新日期：2012-02-01 00:00:00

abstract：:An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwh...

journal_title：Journal of microbiology and biotechnology

authors： Joo HS,Choi JW

更新日期：2012-01-01 00:00:00

abstract：:A novel cry2Ab gene was cloned and sequenced from the indigenous isolate of Bacillus thuringiensis subsp. kurstaki. This gene was designated as cry2Ab25 and its sequence revealed an open reading frame of 1,902 bp encoding a 633 aa protein with calculated molecular mass of 70 kDa and pI value of 8.98. The amino acid se...

journal_title：Journal of microbiology and biotechnology

authors： Sevim A,Eryüzlü E,Demirbağ Z,Demir I

更新日期：2012-01-01 00:00:00

abstract：:In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which th...

journal_title：Journal of microbiology and biotechnology

authors： Mashhadi-Karim M,Azin M,Gargari SL

更新日期：2011-12-01 00:00:00

abstract：:Filamentous fungi colonizing rice straw were collected from 11 different sites in Korea and were identified based on characterization of their morphology and molecular properties. The fungi were divided into 25 species belonging to 16 genera, including 14 ascomycetes, one zygomycete, and one basidiomycete. Fungal cell...

journal_title：Journal of microbiology and biotechnology

authors： Lee S,Jang Y,Lee YM,Lee J,Lee H,Kim GH,Kim JJ

更新日期：2011-12-01 00:00:00

abstract：:A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatchery-reared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained f...

journal_title：Journal of microbiology and biotechnology

authors： Jang IK,Suriakala K,Kim JS,Meng XH,Choi TJ

更新日期：2011-11-01 00:00:00

abstract：:In this study, the chromosome-encoded pcuRCAXB genes that are required for p-cresol degradation have been identified by using a newly constructed green fluorescent protein (GFP)-based promoter probe transposon in the long-chain alkylphenol degrader Pseudomonas alkylphenolia. The deduced amino acid sequences of the gen...

journal_title：Journal of microbiology and biotechnology

authors： Cho AR,Lim EJ,Veeranagouda Y,Lee K

更新日期：2011-11-01 00:00:00

abstract：:Optimal conditions for a high cell density fermentation were investigated in a recombinant Escherichia coli producing salmosin, a platelet aggregation inhibitor. The optimized carbon and nitrogen sources were glycerol 10 g/l, yeast extract 30 g/l, and bacto-tryptone 10 g/l, yielding the dry cell weight (DCW) of 10.61 ...

journal_title：Journal of microbiology and biotechnology

authors： Seo MJ,Choi HJ,Chung KH,Pyun YR

更新日期：2011-10-01 00:00:00

abstract：:Several isolates of Bacillus thuringiensis (Bt) were screened for the vegetative insecticidal protein (Vip) effective against sap-sucking insect pests. Screening results were based on LC(50) values against cotton aphid (Aphis gossypii), one of the dangerous pests of various crop plants including cotton. Among the isol...

journal_title：Journal of microbiology and biotechnology

authors： Sattar S,Maiti MK

更新日期：2011-09-01 00:00:00